Request QuoteCatalog Number: xP002791CHSize: 0.2-1mg

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Recombinant Serine/threonine-protein kinase B-raf (BRAF)

Recombinant Serine/threonine-protein kinase B-raf (BRAF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP002791CHYeast1mgQuote
EP002791CHE. coli1mgQuote
BP002791CHBaculovirus200ugQuote
MP002791CHMammalian Cell200ugQuote

Protein Information

SpeciesGallus gallus (Chicken)
UniProt IDQ04982
Gene NameBRAF; aka: RMIL
Protein NameSerine/threonine-protein kinase B-raf
Region Expressed1-806
Expression Tag6xHis
Purity>90%
AA SequenceMAALSSGSSAEGASLFNGDMEPEPPPPVLGACYAGSGGGDPAIPEEVWNIKQMIKLTQEH IEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTV ASSSSSSLSVAPSSLSVYQNPTDMSRNNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDS LKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRK TFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPI SQEETTLGETTPASGSYPSVPPSDSVGPPILPSPSPSKSIPIPQPFRPADEDHRNQFGQR DRSSSAPNVHINTIEPVNIDDLIRDQGVRGEGAPLNQLMRCLRKYQSRTPSPLLHSVPSE IVFDFEPGPVFRGSTAGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKT LGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKN EVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQT AQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWM APEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSK VRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQT EDFSLYACASPKTPIQAGGYGGFPVH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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