$786.60Catalog Number: MAB179-100ugSize: 100ug

SARS Spike Antibody

SARS-Spike protein, Monoclonal Antibody
Monoclonal Antibody

Monoclonal antibodies provide the best target specificity and affinity. It is also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.

Antigen

The antibody was developed using a synthetic peptide from the putative SARS Spike glycoprotein(Accession no. NP_828851) corresponding to amino acids 19-35.

Applications

This monoclonal antibody can be used for detection of SARS Spike protein in western blot analysis at 1-3 ug/ml. The antibody was tested on a cell line transfected with full-length cDNA coding for the SARS Spike protein with a predicted molecular weight of 139 kDa.

Background

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Contents

100 ug in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide.

Isotype

Mouse IgG1,k.

Storage

Store at 4C, stable for 6 months. For long-term storage, store at -200C.
Western blot analysis of SARS Spike in
(a) transfected mouse melanoma cell lysate and (B) untransfected cell lysate.

Type

Mouse antibody Monoclonal.

Quantity

$786.60

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