Request QuoteCatalog Number: xP518862RLDSize: 0.2-1mg

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Recombinant RNA polymerase sigma factor rpoD (rpoD)

Recombinant RNA polymerase sigma factor rpoD (rpoD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518862RLDYeast1mgQuote
EP518862RLDE. coli1mgQuote
BP518862RLDBaculovirus200ugQuote
MP518862RLDMammalian Cell200ugQuote

Protein Information

SpeciesRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
UniProt IDD5AQI9
Gene NamerpoD; Locus:RCAP_rcc03054
Protein NameRNA polymerase sigma factor rpoD
Region Expressed1-674
Expression Tag6xHis
Purity>90%
AA SequenceMAAKDIDDTKPDTAADEDASFDMSQAAVKRMIGEAKERGYITIDQLNAVMPPETVSGEQI EDVMSMLSEMGINVVEGEEVEESEGGEVVETGSGSREIAVAGAAGETLDRTDDPVRMYLR EMGSVELLSREGEIAIAKRIEAGRNTMIAGLCESPLTFQAITIWRDELLSEEILLRDVID LEATFGRSLDGDEGMEGMEGIEGPVVEGLDLETAEGAAPAARRPASDEPEYDADGNPISR IDEEEDDDDSSNMSLAAMEAALKPKVLETLELIARDYAKLAEMQDLRMSATLNEDGTFSV AEEAAYQKLRSEIVLLVNELHLHNNRIEALIDQLYGINRKIMSIDSGMVKLADAARINRR EFIDEYRGYELDPTWMDRMSAKPARAWVTLFEKSRDKVEDLRHEMAQVGQYVGVDISEFR RIVNQVQKGEKEARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKF EYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPT PEELAEKLQMPLEKVRKVMKIAKEPISLETPIGDEEDSQLGDFIEDKNAILPLDSAIQEN LKETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLK HPSRSRKLRSFLDQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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