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Recombinant RNA polymerase sigma-54 factor (rpoN)

Recombinant RNA polymerase sigma-54 factor (rpoN) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP522142RLDYeast1mgQuote
EP522142RLDE. coli1mgQuote
BP522142RLDBaculovirus200ugQuote
MP522142RLDMammalian Cell200ugQuote

Protein Information

SpeciesRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
UniProt IDD5ANH9
Gene NamerpoN; aka: nifR4, ntrA; Locus:RCAP_rcc00568
Protein NameRNA polymerase sigma-54 factor
Region Expressed1-428
Expression Tag6xHis
Purity>90%
AA SequenceMELAQTLSQRQTMQMAGQMLHSLAILGMSSQDLSEHLTEQATSNPFLTYRAPPAFIARGG EDFDAVAAVAAHKPSLMAHVVDQIEMAFTETPDRLLALRFAEALEPSGWLGQSLDSIALA AGVSLSRAESMLAVLQGFEPTGLFARDLSDCLILQAREADILTWEVETLIRNIRLIAENR LSDLADLCDCDIGDIPEIIKQIRHLNPKPGLAFDHQPTPVFPPDLIAVRGAEGWTVELNR ATSPTITVREDRFADGTADAKARAERRKALAEARALAQALERRGDTLLRTAAVLVARQSA FLDKGPAHLVPLTLEDVASELGLHASTISRAVSGRMIQTQTRALPLRAFFSRAVSTQGGG EAVSRDSALDFVQRTVGGEDPQNPLSDDAIVTLAERAGLRIARRTVAKYRSTLGLASSYE RRRAAAAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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