$238.80Catalog Number: BX1005-20Size: 20 ug

rhIL-6

Recombinant Human Interleukin-6 (rhIL-6)

Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. IL-6 induces growth and terminal differentiation of B cells; secretion of immunoglobulins; differentiation and activation of T cells and macrophages; and the induction of acute-phase response proteins. Many types of cells, including macrophages, T cells, fibroblasts, and endothelial cells, produce IL-6 in response to stimuli such as bacteria, viruses, and other cytokines, particularly IL-1 and tumor necrosis factor, alpha (TNF). Human IL6 is active on both mouse and rat cells, while mouse IL6 has no activity on human cells. Recombinant human IL-6 is a 22 kDa protein containing 189 amino acid residues.

Other Sizes

BX1005-20: 20 ug
BX1005-200: 200 ug
BX1005-1000: 1 mg

Custom size is also available upon request.

Specifications

Lot number: On label
Formulation: Lyophilized powder lyophilized from a volatile buffer (50 mM NH4HCO3 , pH 8.0).
Preservative: None
Molecular Weight: 21 kD
Purity: >97% on 15% SDS-PAGE
Source: Recombinant mature protein expressed in E. coli (189aa)
Sterility: 0.2 um membrane filtered and packaged aseptically
ED50: ND
Endotoxin: ≤0.1 EU/ug protein
QC test: SDS-PAGE

Note: Endotoxin Unit (EU) is determined by Limulus Amebocyte Lysate (LAL) assay.

Reconstitution and use

Reconstitute the contents of the vial using sterile phosphate-buffered saline (PBS) to a concentration no less than 100 ug/ml and aliquot for future use. (If the initial rehydration is too dilute, activity may be lost due to the non-specific adsorption to the container). The solution can then be further diluted to a working stock solution.

If the product is going to be used for applications requiring absolute asepsis, it's best to filter-sterilize the solution using a sterile and non-pyrogenic 0.2 um membrane before use.

Storage and stability

Upon receiving, store the product at -20oC. After reconstitution, store the working aliquots at 2-8oC for no more than 3 months. For extended storage, aliquot the rehydrated solution (≥100 ug/ml) and freeze at -70oC or -20oC. Avoid repeated freezing and thawing. More dilute solutions stored at -20oC will lose activity faster.

Amino Acid Sequence

MAFPAPVPPG EDSKDVAAPH RQPLTSSERI DKQIRYILDG ISALRKETCN KSNMCESSKE ALAENNLNLP KMAEKDGCFQ SGFNEETCLV KIITGLLEFE VYLEYLQNRF ESSEEQARAV QMSTKVLIQF LQKKAKNLDA ITTPDPTTNA SLLTKLQAQN QWLQDMTTHL ILRSFKEFLQ SSLRALRQM

Quantity

$238.80

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