Request QuoteCatalog Number: xP391070GEHSize: 0.2-1mg

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Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA)

Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP391070GEHYeast1mgQuote
EP391070GEHE. coli1mgQuote
BP391070GEHBaculovirus200ugQuote
MP391070GEHMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus thermodenitrificans (strain NG80-2)
UniProt IDA4IJP9
Gene NamerocA; Locus:GTNG_0169
Protein Name1-pyrroline-5-carboxylate dehydrogenase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMVQPYKHEPFTDFTVDANRQAFLAALEKVEAELGREYPLIIGGERVMTEDKITSVNPANK AEVIGRVAKANKELAERAMKTADEAFRTWSRTSPEARADILFRAAAIVRRRKHEFSAWLV KEAGKPWREADADTAEAIDFMEYYGRQMLKLKDGIPVESRPGETNRFFYIPLGVGVVISP WNFPFAIMAGTTVAALVTGNTVLLKPASATPVVAYKFAEVLEEAGLPAGVLNYIPGSGAE VGDYLVEHPRTRFISFTGSRDVGIRIYERAAKVQPGQIWLKRVIAEMGGKDAIVVDKEAD LELAAQSIVASAFGFSGQKCSACSRAIIVEDVYDQVLNRVVELTKQLNVGDPAEQATFMG PVIDQGAYNKIMEYIEIGKQEGRLMTGGEGDDSKGFFIQPTVFADVDPNARIMQEEIFGP VVAFAKARDFDHALEIANNTQYGLTGAVISRNRANLEKARHEFHVGNLYFNRGCTGAIVG YQPFGGFNMSGTDSKAGGPDYLILHMQAKTVSEMF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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