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Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA)

Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP675552Yeast1mgQuote
EP675552E. coli1mgQuote
BP675552Baculovirus200ugQuote
MP675552Mammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
UniProt IDQ4A0E7
Gene NamerocA; Locus:SSP0311
Protein Name1-pyrroline-5-carboxylate dehydrogenase
Region Expressed1-514
Expression Tag6xHis
Purity>90%
AA SequenceMVVNYHNEPSIDFTDSKNVESFKEALKKVKGELNQKIPLVINGEEKFTKDTYQSINPANT TEVIAEVSKATQKDVDDAFEAANEAYKSWKRWSHKDRAEFLIRVAAIIRRRKEEISAVMV YEAGKPWDEAVGDAAEGIDFIEYYARSMMELADGKPVLDREGEHNKYFYKPIGTGVTIPP WNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPLTAYKLMEILEEAGLPKGVVNFVPGDPKE IGDYLVDSVHTHFVTFTGSRATGTRIFERAAKVQDGQQFLKRVIAEMGGKDAIVVDKDID TDLAAESIVSSAFGFSGQKCSACSRAIVHKDVYDEVLEKAVALTKNLTVGNTENNTYMGP VINQKQFDKIKNYIEIGSKEGKLKQGGGTDDATGYFVEPTIIANLKSSDQIMQEEIFGPV VGFVKGKDFEELLEIANDTDYGLTGAVITNNRENWIEAVESYDVGNLYLNRGCTSAVVGY HPFGGFKMSGTDAKTGSPDYLLNFLEQKVVSEMF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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