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Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA)

Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP680621Yeast1mgQuote
EP680621E. coli1mgQuote
BP680621Baculovirus200ugQuote
MP680621Mammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus haemolyticus (strain JCSC1435)
UniProt IDQ4L966
Gene NamerocA; Locus:SH0500
Protein Name1-pyrroline-5-carboxylate dehydrogenase
Region Expressed1-514
Expression Tag6xHis
Purity>90%
AA SequenceMVVPFKNEPGIDFSVQENVERFQKTLEQVKNELGQTLPIVIDGEHITKDDTFDSINPANT SELIAKVSKATKEDVDKAFESSNKAYKAWRQWSHKDRAELLLRVAAIIRRRKEEISAVMV YEAGKPWDEAVGDAAEGIDFIEYYARSMMELADGKPVLDREGEHNKYFYKSIGTGVTIPP WNFPFAIMAGTTLAPVVAGNTVLLKPAEDTVLTAYKLIEILEEAGLPKGVVNFVPGDPKE IGDYLVDSVHTHFVTFTGSRATGTRIYERSAVVQEGQTFLKRVIAEMGGKDAIVVDENID TDLAAESIITSAFGFSGQKCSACSRAIVHSSVYDEVLEKAVALTKELTVGNTVDNTFMGP VINKKQFDKIKKYIEIGGKEGKIEIGGEADDSTGYFIKPTIISGLKSSDQVMQEEIFGPV VGFTKFDNFEEAIEIANDTDYGLTGAVITNNRENWIKAVNEFDVGNLYLNRGCTAAVVGY HPFGGFKMSGTDAKTGSPDYLLNFLEQKVVSEMF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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