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Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA)

Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP309510SKYYeast1mgQuote
EP309510SKYE. coli1mgQuote
BP309510SKYBaculovirus200ugQuote
MP309510SKYMammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus aureus (strain N315)
UniProt IDP99076
Gene NamerocA; Locus:SA2341
Protein Name1-pyrroline-5-carboxylate dehydrogenase
Region Expressed1-514
Expression Tag6xHis
Purity>90%
AA SequenceMVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGEKIFKDDKIKSINPADT SQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELMLRVSAIIRRRKAEIAAIMV YEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDREGEHNKYFYKSIGTGVTIPP WNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLMEILEEAGLPKGVVNFVPGDPKE IGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQNFLKRVIAEMGGKDAIVVDENID TDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYDEVLEKSIKLTKELTLGNTVDNTYMGP VINKKQFDKIKNYIEIGKEEGKLEQGGGTDDSKGYFVEPTIISGLKSKDRIMQEEIFGPV VGFVKVNDFDEAIEVANDTDYGLTGAVITNNREHWIKAVNEFDVGNLYLNRGCTSAVVGY HPFGGFKMSGTDAKTGSPDYLLHFLEQKVVSEMF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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