Request QuoteCatalog Number: xP510623SXFSize: 0.2-1mg

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Recombinant Pup--protein ligase (pafA)

Recombinant Pup--protein ligase (pafA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP510623SXFYeast1mgQuote
EP510623SXFE. coli1mgQuote
BP510623SXFBaculovirus200ugQuote
MP510623SXFMammalian Cell200ugQuote

Protein Information

SpeciesSanguibacter keddieii (strain ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74)
UniProt IDD1BHT7
Gene NamepafA; Locus:Sked_20850
Protein NamePup--protein ligase
Region Expressed1-455
Expression Tag6xHis
Purity>90%
AA SequenceMSTRRIFGLETEYGITCAAPPGQQGLSADEVARYLFRKVVAWGRSSNVFLRNGSRLYLDV GSHPEYATAECDDIRQLITYDRGGERILEGLVADAQQRLELEGVPGTIHLFKNNTDSAGN SYGCHENYLVRRQGDFTRLADILVPFLITRQVLTGAGKILTTPAGATFCLSQRADHIWES VSSATTRSRPIINTRDEPHADAELYRRLHVIVGDSSMAEPTAMLKVGATDMVLRMIEAGV PMRDMALENPMRAIREISHDMTGKAPITLASGRTVTAVDLQQEYLVKVRDFVESTGEVSD TDRKVFDLWERGLLALQSGDLTSVERELDWVIKHRLVRRYQEKHSLELSDPRIARLDLAY HDISRTEGLYNLLAARGLVERVTSDVEVLESTAVPPQTTRARLRGEFVRRAQEAKRDYTV DWVHLKLNDQAQRTVLCKDPFQSVDERVDRLIESM
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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