Request QuoteCatalog Number: xP513942GGYSize: 0.2-1mg

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Recombinant Pup--protein ligase (pafA)

Recombinant Pup--protein ligase (pafA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513942GGYYeast1mgQuote
EP513942GGYE. coli1mgQuote
BP513942GGYBaculovirus200ugQuote
MP513942GGYMammalian Cell200ugQuote

Protein Information

SpeciesGordonia bronchialis (strain ATCC 25592 / DSM 43247 / JCM 3198 / NCTC 10667) (Rhodococcus bronchialis)
UniProt IDD0LDT3
Gene NamepafA; Locus:Gbro_2465
Protein NamePup--protein ligase
Region Expressed1-452
Expression Tag6xHis
Purity>90%
AA SequenceMQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLQNGARLYLDVGS HPEYATAECDSLIQLINHDRAGELVLEDLLVDAEQRLSDEGIGGDIYLFKNNTDSAGNSY GCHENYLVVRAGEFSRISDVLLPFLVTRQLICGAGKVLQTPKAATFCLSQRAEHIWEGVS SATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSEMTTLLKVGSAALVLEMIEAGVVF RDFALDNPIRAIREASHDPTGRRPVRLAGGRQASALDIQREYHARAVEHMTNRRPDPEMD MVVDLWGRMLDAVERDDFSKVDTEVDWVIKRKLFQRYQDKYSMELSDPKIAQLDLAFHDI KRGRGVFDVLSRKGLVTRATTDEAIATAVNEPPQTTRAKLRGDFISAAQKAGRDFTVDWV HLKLNDQAQRTVLCKDPFRSVDERVDRLIASM
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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