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Recombinant Pup--protein ligase (pafA)

Recombinant Pup--protein ligase (pafA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP512128FOLYeast1mgQuote
EP512128FOLE. coli1mgQuote
BP512128FOLBaculovirus200ugQuote
MP512128FOLMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces scabies (strain 87.22) (Streptomyces scabiei)
UniProt IDC9Z4D4
Gene NamepafA; Locus:SCAB_73511
Protein NamePup--protein ligase
Region Expressed1-453
Expression Tag6xHis
Purity>90%
AA SequenceMDRRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGS HPEYATPECDNVTELVTHDKAGERILEGLLVDAERRLHEEGIAGDVYLFKNNTDSAGNSY GCHENYLVARHGEFSRLADILIPFLVTRQLLCGAGKVLQTPRGAVYCVSQRAEHIWEGVS SATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSETTMLLKVGATDLVLRMIEAGTVM RDLTLENPIRAIREVSHDITGRRKVRLASGREASALEVQREYYEKAVDFVERRGIRTGTV EQVLELWGRTLDAIEAEDLDRIGTEIDWVMKYKLIERYRAKHNMTMSHPRVAQIDLAYHD IHRRRGLYYLLERKGQATRICNDLKIFEGKSVPPQTTRARLRGDFIRRAQEQRRDFTVDW VHLKLNDQAQRTVLCKDPFRSVDDRVEKLIAGM
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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