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Recombinant [Protein-PII] uridylyltransferase (glnD)

Recombinant [Protein-PII] uridylyltransferase (glnD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP659627Yeast1mgQuote
EP659627E. coli1mgQuote
BP659627Baculovirus200ugQuote
MP659627Mammalian Cell200ugQuote

Protein Information

SpeciesNitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
UniProt IDQ3SWE0
Gene NameglnD; Locus:Nwi_0133
Protein Name[Protein-PII] uridylyltransferase
Region Expressed1-925
Expression Tag6xHis
Purity>90%
AA SequenceMVLPTTKDATAAGAGFDTVRIIGDIDALAEKHAGHEDVFRSAVSRLLKAELAKVRDAAQA KLLRDRHGRHCAEWLCFVQDEIIRLSFSAATRHLYHSPIPSDGERMAVVATGGYGRGLMA PESDIDLLFILPYKQTAWGEQVAEAILYCLWDMGLNVGHATRSVNESIRQARRDMTVRTG ILEARFLTGDRALYDELISRFDTEVVQGTAAEFVAAKLAEREERHHRAGQSRYLVEPNVK DGKGGLRDLHTLFWIAKYVYRVRESHELLRRNVFDVREYRTFRRCADFLWSVRCNLHFAT GRAEERLSFDLQREIAVRLGYTSHPGMQDVERFMKHYFLTAKDVGDLTAILCAKLEDQQA KPAPVLSRAMSKPPGAEVRRVPDSDDFIIDNNRINLAAPDLFKRDPVNLIRLFRLAQKNN LAFHPDALRMVRRSRRLINAQLREDPESNRLFIEILTSNDAETVLRRMNETGVLGEFIRA FGKIVSMMQFNMYHHYTVDEHLIRCIGILQDIERGDNDEVALAGELMRTINPEHRPVIYI ATLLHDVAKGRPEDHSIAGARVARRLCPRLGFNAADTELVAWLIEQHLTMSKVAQSRDLS DRKTIENFAAVVQSVERMKLLTILTTADIRGVGPGVWNGWKAQLLRTLYYETEPVLTGGF SEVNRAQRMAAAEAEFRAAFTEWSGHELNAYIARHYPAYWLKVDLEHKIRHARFLRASEQ SGRKLNINVGFDEARGVTELTILAADHPWLLSIIAGACASAGANIVDAQIYTTTDGQALD TIAISREYERDEDEGRRAARIAEIIEQVLEGRLRLPDVMPSRAAGKRLRPFVVEPKVTIN NQWSDRHTMIEVSGLDRPGLLFQLTTAISKLNLNIASAHVATFGERARDVFYVTDLLGAR ITAPTRQAAIKRALVHLLASGNTAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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