$90.00Catalog Number: PYT322-10ugSize: 10ug

Prolactin Rat

Prolactin Rat Recombinant

Prolactin Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids and having a molecular mass of 22.6 kDa.
The Prolactin is purified by proprietary chromatographic techniques.

Accession

P01237

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Val-Cys.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be < 0.065 ng/ml corresponding to a specific activity of 15.4MUnits/mg.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution with 10mM sodium Phosphate buffer pH=8 and 50mM Nacl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Prolactin should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Mammotropin, Luterotropic hormone, Lutetropin, PRL.

Quantity

$90.00

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