P16471

The sequence of the first six N-terminal amino acids was determined to be Ala-Gly-Lys-Pro-Glu-Ile.

Activity is determined by the dose-dependant inhibition of Prolactin stimuled proliferation of Nb2 cells and by high affinity binding of ovine Prolactin and other lactogenic hormones in 1:1 molar ratio.

The Prolactin Receptor was lyophilized from a concentrated (0.4mg/ml) solution with 0.0045mM NaHCO₃.

Sterile filtered white lyophilized powder.

UV spectroscopy at 280 nm using the absorbency value of 2.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(c) Gel filtration at pH 8 under non denaturative conditions.

It is recommended to reconstitute the lyophilized PRLR in sterile 18M-cm H₂O not less than 100ug/ml and not more than 1 mg/ml, which can then be further diluted to other aqueous solutions.

Escherichia Coli.

Lyophilized PRL-R although stable at room temperature for 1-2 weeks, should be stored desiccated below -18^oC or preferably even at -80^oC to prevent dimer formation. Upon reconstitution PRL-R should be stored sterile at 4^oC between 2-7 days and for future use below -18^oC. For long term storage at 4^oC it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles as they cause oligomerization of the protein.

PRL-R, hPRLrI.