Human P38γ (MAPK12) Xpress Clones
Mitogen-activated protein kinases (MAPKs) cascade relays extracellular signals from cell membrane to the nucleus to induce intracellular responses and to regulate many aspects of cell physiology. These cascades, including JNK, ERK and p38 pathways, consist of distinct members of regulatory enzymes that serially activate one another in response to growth factors, cytokines and other mitogenic stimuli, and lead to activation or inactivation of transcription factors. Proinflammatory cytokine and microbial products activate p38 (SAPK2, MAPK12) gene regulating pathway and cause the expression of multiple cytokine genes including IL-1, IL-6 and TNF alpha. There're four known p38 isoforms (α, β, γ and δ) that share about 60% homology. Gene Name: Homo sapiens mitogen-activated protein kinase 12 (MAPK12), (Nicknames: p38 gamma, p38r, p38g)
Specifications
| Materials Provided: | 20 ug transfection ready plasmids in 40ul TE buffer. |
| Antibiotic Selections: | Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells |
| Cloning vector: | pMEV-2HA |
| 5' cloning site: | Xho I |
| 3' cloning site: | Xba I |
| GenBank#: | NM_002969 |
| Mutations: | K56M; T183E, Y185E; T183A, Y185A |
Maintenance of plasmid clones
Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
Human P38γ (MAPK12) protein sequence
Human P38γ (MAPK12) nucleotide sequence
About Xpress Clones
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
