$678.00Catalog Number: BXJ1106-aaSize: 20 ug

pMEVHA-JNKK1-AA

Human JNKK1 (MAP2K4) Xpress Clones

Mitogen-activated protein kinases (MAPKs) cascade relays extracellular signals from cell membrane to the nucleus to induce intracellular responses and to regulate many aspects of cell physiology. These cascades, including JNK, ERK and p38 pathways, consist of distinct members of regulatory enzymes that serially activate one another in response to growth factors, cytokines and other mitogenic stimuli, leading to (in)activation of transcription factors. Like ERK1/2 and p38, the JNK activation requires dual phosphorylation on tyrosine and threonine residues within a conserved TPY motif. Activated JNKK1/2 can preferably phosphorylate and activate JNKs, with JNKK2 more specific to JNK.

Includes and available separately

BXJ1106-5:20 ug each
BXJ1106-wt:20 ug
BXJ1106-km:20 ug
BXJ1106-ee:20 ug
BXJ1106-aa:20 ug
BXJ1106-kmaa:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Kpn I
GenBank#:NM_003010
Mutations:K131M; S257E, T261E; S257A, T261A; K131M, S257A, T261A

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human JNKK1 (MAP2K4) protein sequence

Human JNKK1 (MAP2K4) nucleotide sequence

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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