Human YAP2 Xpress Clones
Human YAP genes were clones initially using a chicken YAP as the probe. It is a co-activator of transcription factors. When phosphoraylated by Lats or other upstream kinases, YAP protein translocates into the cytoplasm and has less transcriptional activation effect. YAP is one component of Hippo pathway involved in cell proliferation, apoptosis, cancer, cell contact inhibition and organ size control.
YAP2 contains five HXRXXS motifs, which is the recognition sequence for upstream kinase Lats2. In YAP2-5SA mutant, all five groups of serine residues (including S127) are mutated to alanine. This mutant protein can no longer be phosphorylated so more of it remains in the nucleus. As predicted, this mutant has relatively higher basal activity in a reporter assay.
YAP2-5SA-ΔC (H1016c), which is the YAP2-5SA nucleus-localizing form with a deletion of the C-terminal transcription activation domain. This mutant YAP2 is insensitive to the Hippo pathway-induced cytoplasmic translocation and cannot activate gene expression, and therefore may act as a dominant-negative form. Overexpression of this mutant restored contact inhibition in a human cancer cell
Gene Name: Homo sapiens yes-associated protein 2 (YAP2); AKA: YAP; YAP2; YAP65; YKI.
Includes and available separately
| BXH1016-3: | 20 ug each |
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| BXH1016-wt: | 20 ug |
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| BXH1016-5sa: | 20 ug |
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| BXH1016-delc: | 20 ug |
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Custom size is also available upon request.
Specifications
| Materials Provided: | 20 ug transfection ready plasmids in 40ul TE buffer. |
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| Antibiotic Selections: | Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells |
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| Cloning vector: | pMEV-2HA |
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| 5' cloning site: | Bam HI |
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| 3' cloning site: | Sal I |
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| GenBank#: | AY316529 |
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| Mutations: | S61A,S109A, S127A, S128A, S131A, S163A, S164A;
S61A,S109A, S127A, S128A, S131A, S163A, S164A, G292 to L488 deletion |
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Maintenance of plasmid clones
Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
Human YAP2 protein sequence
Human YAP2 nucleotide sequence
About Xpress Clones
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
