Human p53 Xpress Clones
The p53 protein is a nuclear phosphoprotein of 53 kDa. It is a tumor suppressor protein critical for regulation of the cell cycle in response to genotoxic insults such as radiation or chemicals resulting in DNA damages. The highly conserved vertebrate p53 gene is one of the most frequently mutated genes found in human cancers, being either lost or mutated in over half of all human tumors. It has therefore become the center of intensive study ever since the link of p53 mutations with various human cancers was realized. The expression level of several important genes including p21, MDM2, GADD45 (CHOP), Bax and cyclin G was modulated by p53. Among many other roles, it is involved in arresting the cell cycle at G1 phase upon DNA damages.
The native p53 protein is a tetramer in solution and functions as a transcription factor that binds to specific DNA sequences. Many P53 mutations, especially those lie within the DNA-binding domain (so-called hot-spot mutations), show dominance over wild type protein. Replacement of valine 143, one of the hot-spot residues, results in a mutant (V143A) with a transdominant-negative effect on several activities of wild type p53. V143A is also a temperature-sensitive mutant: it shows DNA binding and transcriptional activity at 32oC, but it is inactive at 37oC.
Includes and available separately
| BXP1035-2: | 20 ug each |
| BXP1035-wt: | 20 ug |
| BXP1035-dn: | 20 ug |
Custom size is also available upon request.
Specifications
| Materials Provided: | 20 ug transfection ready plasmids in 40ul TE buffer. |
| Antibiotic Selections: | Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells |
| Cloning vector: | pMEV-2HA |
| 5' cloning site: | Bam HI |
| 3' cloning site: | Eco RI |
| GenBank#: | NM_000546 |
| Mutations: | DN: V143A |
Maintenance of plasmid clones
Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
Human p53 protein sequence
MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDI
EQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQ
KTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDST
PPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGN
LRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRP
ILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELP
PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALEL
KDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
Human p53 nucleotide sequence
ATGGAGGAGC CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA
AACATTTTCA GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC
CCTTGCCGTC CCAAGCAATG GATGATTTGA TGCTGTCCCC GGACGATATT
GAACAATGGT TCACTGAAGA CCCAGGTCCA GATGAAGCTC CCAGAATGCC
AGAGGCTGCT CCCCGCGTGG CCCCTGCACC AGCGACTCCT ACACCGGCGG
CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG
AAAACCTACC AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG
GACAGCCAAG TCTGTGACTT GCACGTACTC CCCTGCCCTC AACAAGATGT
TTTGCCAACT GGCCAAGACC TGCCCTGTGC AGCTGTGGGT TGATTCCACA
CCCCCGCCCG GCACCCGCGT CCGCGCCATG GCCATCTACA AGCAGTCACA
GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG CGCTGCTCAG
ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT
TTGCGTGTGG AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT
GGTGCCCTAT GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT
ACAACTACAT GTGTAACAGT TCCTGCATGG GCGGCATGAA CCGGAGGCCC
ATCCTCACCA TCATCACACT GGAAGACTCC AGTGGTAATC TACTGGGACG
GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA GACCGGCGCA
CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC
CCAGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA
GCCAAAGAAG AAACCACTGG ATGGAGAATA TTTCACCCTT CAGATCCGTG
GGCGTGAGCG CTTCGAGATG TTCCGAGAGC TGAATGAGGC CTTGGAACTC
AAGGATGCCC AGGCTGGGAA GGAGCCAGGG GGGAGCAGGG CTCACTCCAG
CCACCTGAAG TCCAAAAAGG GTCAGTCTAC CTCCCGCCAT AAAAAACTCA
TGTTCAAGAC AGAAGGGCCT GACTCAGACT GA
About Xpress Clones
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
