Human OCT3/4 Xpress Clone
Human POU class 5 homeobox 1 (POU5F1)
Genes such as OCT3/4, SOX2, NANOG, LIN28 and KLF4 are highly expressed in undifferentiated embryonic stem cells and progenitor cells. They are important for stem cells to have their special properties or "stemness", and hence are sometimes called "Stemness Genes". Tremendous advances have been achieved recently in induced pluripotent stem (iPS) cells from mouse and human somatic cells. These cells have enormous potentials in curing many human diseases. To facilitate stem cell research and exploration, we offer all the "Stemness Genes" that have been used to induce iPS: OCT3/4, SOX2, NANOG, LIN28, c-Myc and KLF4.
OCT3/4, SOX2, c-Myc and KLF4 were initially used to generate induced pluripotent stem cells (iPSC) from mouse adult cells (Takahashi & Yamanaka, 2007). Human somatic cells were later induced with the same set of genes (Park et al., 2008), and with a different set of genes consisting OCT3/4, SOX2, NANOG and LIN28 by Yu et al (2007).
Specifications
| Materials Provided: | 20 ug transfection ready plasmids in 40ul TE buffer. |
|---|
| Antibiotic Selections: | Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells |
|---|
| Cloning vector: | pMEV-2HA |
|---|
| 5' cloning site: | Bam HI |
|---|
| 3' cloning site: | Xba I |
|---|
| GenBank#: | NM_002701 |
|---|
| Mutations: | None |
|---|
Maintenance of plasmid clones
Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. If received in lyophilized form, the plasmid can be reconstituted in buffer of choice, preferably in 40ul of TE. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
Human OCT3/4 protein sequence
Human OCT3/4 nucleotide sequence
Select references
- Takahashi K. & Yamanaka S. 2006. Cell, 126(4): 663-76
- Takahashi K. et al., 2007, Cell, 131: 861-72
- Yu J, et al., 2007, Science, 318: 1917-20
- Meissner A. et al., 2007. Nat Biotechnol. 25: 1177-81
- Ramalho-Santos M et al., 2007, Cell stem Cell, 1: 245-7
- Park IH, et al., 2008. Nature, 451: 141-6
- Aoi T. et al., 2008, Science, Epub 14 Feb. 2008, 10.1126/Science.1154884
- Lowry WE et al., 2008. PNAS USA, 105: 2883-8
- Nakagawa M., 2008. Nat Biotechnol. 26: 101-6
About Xpress Clones
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
