Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY
RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC
VFAINNSKSF ADINLYREQI KRVKDSDDVP MVLVGNKCDL
PTRTVDTKQA HELAKSYGIP FIETSAKTRQ GVEDAFYTLV
REIRQYRMKK LNSSDDGTQG CMGLPCVVM
ATGACTGAGT ACAAACTGGT GGTGGTTGGA GCAGGTGGTG TTGGGAAAAG
CGCACTGACA ATCCAGCTAA TCCAGAACCA CTTTGTAGAT GAATATGATC
CCACCATAGA GGATTCTTAC AGAAAACAAG TGGTTATAGA TGGTGAAACC
TGTTTGTTGG ACATACTGGA TACAGCTGGA CAAGAAGAGT ACAGTGCCAT
GAGAGACCAA TACATGAGGA CAGGCGAAGG CTTCCTCTGT GTATTTGCCA
TCAATAATAG CAAGTCATTT GCGGATATTA ACCTCTACAG GGAGCAGATT
AAGCGAGTAA AAGACTCGGA TGATGTACCT ATGGTGCTAG TGGGAAACAA
GTGTGATTTG CCAACAAGGA CAGTTGATAC AAAACAAGCC CACGAACTGG
CCAAGAGTTA CGGGATTCCA TTCATTGAAA CCTCAGCCAA GACCAGACAG
GGTGTTGAAG ATGCTTTTTA CACACTGGTA AGAGAAATAC GCCAGTACCG
AATGAAAAAA CTCAACAGCA GTGATGATGG GACTCAGGGT TGTATGGGAT
TGCCATGTGT GGTGATGTAA
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
