Mouse MEKK1 Xpress Clones
MAPK pathways play a key role in mediating cell growth and apoptosis. Among the components of the MAPK family, mitogen-activated protein kinase kinase kinase (MEKK1) is a 196-kDa serine-threonine kinase that was first cloned from a cDNA library derived from NIH 3T3 cells by virtue of its homology to Ste11 and Byr2. MEKK1 is activated in response to cytokines and various stresses, with GSK3 beta as one of the direct activators. MEKK1 preferentially activates the JNK pathway and also influences the activity of the ERK pathway, while it has little or no effect on the p38-MAPK pathways. MEKK1 has been reported to be involved in cardiac hypertrophy, mitochondrial permeability and apoptosis, focal adhesion and cell migration, activating C/EBP-beta-dependent gene expression in response to IFN-gamma.
MEKK1-KD is the c-terminal protein kinase domain of mouse MEKK1, consisting of amino acid residues 375-672. It is constitutively active and has been used to activate MAPK pathways in transfection assays. Conserved in all protein kinases, lysine 452 in MEKK1 is catalytically essential. Changing it to a methionine, as is the case in MEKK1-K452M mutant, results in a mutant protein essentially lacking kinase activity.
Includes and available separately
| BXP1045-2: | 20 ug each |
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| BXP1045-kd: | 20 ug |
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| BXP1045-k452m: | 20 ug |
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Custom size is also available upon request.
Specifications
| Materials Provided: | 20 ug transfection ready plasmids in 40ul TE buffer. |
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| Antibiotic Selections: | Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells |
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| Cloning vector: | pMEV-2HA |
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| 5' cloning site: | Bam HI |
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| 3' cloning site: | Eco RI |
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| GenBank#: | NM_011945; L13103; AF117340 |
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| Mutations: | DN: K452M |
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Maintenance of plasmid clones
Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
Mouse MEKK1 protein sequence
MSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWL
KGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREE
IRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKES
VVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAAR
LASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMAC
AKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVAVRCLELQPQ
DRPPSRELLKHPVFRTTW
Mouse MEKK1 nucleotide sequence
ATGTCAGCGT CTCAGGATGC CCTCCCCATC GTCCCTCAGC TGCAGGTGGA
AAATGGAGAA GATATTATCA TCATTCAGCA GGACACACCA GAAACTCTTC
CAGGACATAC CAAAGCGAAA CAGCCTTACA GAGAAGACGC TGAGTGGCTG
AAAGGCCAGC AGATAGGCCT CGGAGCATTT TCTTCCTGTT ACCAAGCACA
GGATGTGGGG ACTGGGACTT TAATGGCTGT GAAACAGGTG ACGTACGTCA
GAAACACATC CTCCGAGCAG GAGGAGGTGG TGGAAGCGTT GAGGGAAGAG
ATCCGGATGA TGGGTCACCT CAACCATCCA AACATCATCC GGATGCTGGG
GGCCACGTGC GAGAAGAGCA ACTACAACCT CTTCATTGAG TGGATGGCGG
GAGGATCTGT GGCTCACCTC TTGAGTAAAT ACGGAGCTTT CAAGGAGTCA
GTCGTCATTA ACTACACTGA GCAGTTACTG CGTGGCCTTT CCTATCTCCA
CGAGAACCAG ATCATTCACA GAGACGTCAA AGGTGCCAAC CTGCTCATTG
ACAGCACCGG TCAGAGGCTG AGAATTGCAG ACTTTGGAGC TGCTGCCAGG
TTGGCATCAA AAGGAACCGG TGCAGGAGAG TTCCAGGGAC AGTTACTGGG
GACAATTGCA TTCATGGCGC CTGAGGTCCT AAGAGGTCAG CAGTATGGTA
GGAGCTGTGA TGTATGGAGT GTTGGCTGCG CCATTATAGA AATGGCTTGT
GCAAAACCAC CTTGGAATGC AGAAAAACAC TCCAATCATC TCGCCTTGAT
ATTTAAGATT GCTAGCGCAA CTACTGCACC GTCCATCCCG TCACACCTGT
CCCCGGGTCT GCGCGACGTG GCCGTGCGCT GCTTAGAACT TCAGCCTCAG
GACCGGCCTC CGTCCAGAGA GCTGCTGAAA CATCCGGTCT TCCGTACCAC
GTGGTAG
About Xpress Clones
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
