$678.00Catalog Number: BXP1030-dnSize: 20 ug

pMEV2HA-MEK1-DN

Human MEK1 Xpress Clone, dominant negative

MEK1 and MEK2 are members of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. MEKs are involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Several residues are essential for MEK to function: K97 for its catalytic activity, S218 and S222 for activation by upstream activators. The dominant negative mutant provided (P1030b) contains 3 point mutations (K97R; S218A; S222A) and can neither be phosphorylated by its activators nor phosphorylate its downstream effectors (ERKs). The constitutively active mutant (P1030c) contains 2 point mutations (S218E and S222E) and a deletion of amino acid residues 31-52. The resulting protein is ~400 times more active than recombinant MEK1 wild type expressed in E. coli, and is active without the need of being phosphorylated by its upstream activators.

Includes and available separately

BXP1030-4:20 ug each
BXP1030-wt:20 ug
BXP1030-dn:20 ug
BXP1030-ca:20 ug
BXP1030-k97r:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Bam HI
GenBank#:NM_002755
Mutations:DN: K97R, S218A, S222A; CA: del32-51, S218E, S222E; K97R: K97R

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

hMEK1 protein sequence

MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLE
FLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEI
KPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGK
VSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSP
ERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRP
LSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIK
RSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV

hMEK1 nucleotide sequence

ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGACGG
CTCTGCAGTT AACGGGACCA GCTCTGCGGA GACCAACTTG GAGGCCTTGC
AGAAGAAGCT GGAGGAGCTA GAGCTTGATG AGCAGCAGCG AAAGCGCCTT
GAGGCCTTTC TTACCCAGAA GCAGAAGGTG GGAGAACTGA AGGATGACGA
CTTTGAGAAG ATCAGTGAGC TGGGGGCTGG CAATGGCGGT GTGGTGTTCA
AGGTCTCCCA CAAGCCTTCT GGCCTGGTCA TGGCCAGAAA GCTAATTCAT
CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATAAGGG AGCTGCAGGT
TCTGCATGAG TGCAACTCTC CGTACATCGT GGGCTTCTAT GGTGCGTTCT
ACAGCGATGG CGAGATCAGT ATCTGCATGG AGCACATGGA TGGAGGTTCT
CTGGATCAAG TCCTGAAGAA AGCTGGAAGA ATTCCTGAAC AAATTTTAGG
AAAAGTTAGC ATTGCTGTAA TAAAAGGCCT GACATATCTG AGGGAGAAGC
ACAAGATCAT GCACAGAGAT GTCAAGCCCT CCAACATCCT AGTCAACTCC
CGTGGGGAGA TCAAGCTCTG TGACTTTGGG GTCAGCGGGC AGCTCATCGA
CTCCATGGCC AACTCCTTCG TGGGCACAAG GTCCTACATG TCGCCAGAAA
GACTCCAGGG GACTCATTAC TCTGTGCAGT CAGACATCTG GAGCATGGGA
CTGTCTCTGG TAGAGATGGC GGTTGGGAGG TATCCCATCC CTCCTCCAGA
TGCCAAGGAG CTGGAGCTGA TGTTTGGGTG CCAGGTGGAA GGAGATGCGG
CTGAGACCCC ACCCAGGCCA AGGACCCCCG GGAGGCCCCT TAGCTCATAC
GGAATGGACA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT
AGTCAACGAG CCTCCTCCAA AACTGCCCAG TGGAGTGTTC AGTCTGGAAT
TTCAAGATTT TGTGAATAAA TGCTTAATAA AAAACCCCGC AGAGAGAGCA
GATTTGAAGC AACTCATGGT TCATGCTTTT ATCAAGAGAT CTGATGCTGA
GGAAGTGGAT TTTGCAGGTT GGCTCTGCTC CACCATCGGC CTTAACCAGC
CCAGCACACC AACCCATGCT GCTGGCGTCT AA

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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