Human K-Ras 2A Xpress Clones
Ras proteins are members of small GTPase superfamily. They bind GDP/GTP and possess intrinsic GTPase activity. These proteins act as switches to turn on/off their downstream target proteins in response to upstream stimuli because they can alternate between an inactive form bound to GDP and an active form bound to GTP. Activating mutations of KRAS2, including G12V, have been implicated in various malignancies, including lung denocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. On the other hand, S17N is a widely used dominant negative mutant.
Alternative splicing of KRAS-2 gene leads to variants encoding two isoforms that differ in the C-terminal region. KRAS-2A consists of six exons, including exon 4a, which the shorter transcript variant b lacks.
Gene Name: Homo sapiens v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog (KRAS2), transcript variant A. Other Names: Transforming protein p21A, K-Ras 2A, Ki-Ras, c-K-ras
Includes and available separately
| BXP1020-3: | 20 ug each |
|---|
| BXP1020-wt: | 20 ug |
|---|
| BXP1020-sn: | 20 ug |
|---|
| BXP1020-gv: | 20 ug |
|---|
| |
|---|
Custom size is also available upon request.
Specifications
| Materials Provided: | 20 ug transfection ready plasmids in 40ul TE buffer. |
|---|
| Antibiotic Selections: | Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells |
|---|
| Cloning vector: | pMEV-2HA |
|---|
| 5' cloning site: | Bam HI |
|---|
| 3' cloning site: | Xho I |
|---|
| GenBank#: | NM_033360, NP_203524 |
|---|
| Mutations: | S17N; G12V |
|---|
Maintenance of plasmid clones
Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
Human K-Ras 2A protein sequence
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY
RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC
VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL
PSRTVDTKQA QDLARSYGIP FIETSAKTRQ GVDDAFYTLV
REIRQYRLKK ISKEEKTPGC VKIKKCIIM
Human K-Ras 2A nucleotide sequence
ATGACTGAAT ATAAACTTGT GGTGGTTGGA GCTGGTGGCG TAGGCAAGAG
TGCCTTGACG ATACAGCTAA TTCAGAATCA TTTTGTGGAC GAATATGATC
CAACAATAGA GGATTCCTAC AGGAAGCAAG TAGTAATTGA TGGAGAAACC
TGTCTCTTGG ATATTCTCGA CACAGCAGGT CAAGAGGAGT ACAGTGCAAT
GAGGGACCAG TACATGAGGA CTGGGGAGGG CTTTCTTTGT GTATTTGCCA
TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT
AAAAGAGTTA AGGACTCTGA AGATGTACCT ATGGTCCTAG TAGGAAATAA
ATGTGATTTG CCTTCTAGAA CAGTAGACAC AAAACAGGCT CAGGACTTAG
CAAGAAGTTA TGGAATTCCT TTTATTGAAA CATCAGCAAA GACAAGACAG
GGTGTTGATG ATGCCTTCTA TACATTAGTT CGAGAAATTC GAAAACATAA
AGAAAAGATG AGCAAAGATG GTAAAAAGAA GAAAAAGAAG TCAAAGACAA
AGTGTGTAAT TATGTAA
About Xpress Clones
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
