Human IRAK1 Xpress Clones
Interleukin-1 receptor-associated kinases (IRAK), identified as a serine/threonine kinases coprecipitated with IL-1 receptor (IL-1R) in an IL-1 inducible manner, share significant homology to pelle. The mammalian family of IRAK consists of two active kinases, IRAK-1 and IRAK-4; and two inactive kinases, IRAK-2 and IRAK-M. They are implicated in the TLR/IL-1R pathway to both IKK and MAPKs activation, leading to the activation of transcription factors like NF-κB and AP-1.
Human IRAK-1 gene (NM_001569) encodes a protein of 90kDa in a wide variety of tissues Like other IRAKs, IRAK-1 is a multidomain protein, consisting of N-terminal death domain (DD) and a central kinase domain (KD). Mutation in the ATP binding pocket, K239A renders the enzyme catalytically inactive. Overexpression of the kinase deficient IRAK-1 did not interfere with IL-1-induced NF-kB activation but strongly induced NF-kB activation on its own. The kinase activity of IRAK-1 is complementary to kinase activity lacking IL-1Rs or TLRs to transduce their signals through homophilic interaction of TIR and DD domain.
Gene Name: Homo sapiens interleukin-1 receptor-associated kinase 1.
Includes and available separately
| BXP1050-2: | 20 ug each |
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| BXP1050-wt: | 20 ug |
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| BXP1050-ka: | 20 ug |
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Custom size is also available upon request.
Specifications
| Materials Provided: | 20 ug transfection ready plasmids in 40ul TE buffer. |
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| Antibiotic Selections: | Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells |
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| Cloning vector: | pMEV-2HA |
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| 5' cloning site: | Bam HI |
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| 3' cloning site: | Bam HI |
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| GenBank#: | NM_001569 |
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| Mutations: | K239A |
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Maintenance of plasmid clones
Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
Human IRAK1 protein sequence
MAGGPGPGEPAAPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRLCE
RSGQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAWHPPAPLPSPGTTAPRPSS
IPAPAEAEAWSPRKLPSSASTFLSPAFPGSQTHSGPELGLVPSPASLWPPPPSPAPSSTK
PGPESSVSLLQGARPSPFCWPLCEISRGTHNFSEELKIGEGGFGCVYRAVMRNTVYAVKR
LKENADLEWTAVKQSFLTEVEQLSRFRHPNIVDFAGYCAQNGFYCLVYGFLPNGSLEDRL
HCQTQACPPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLTPKLGDFG
LARFSRFAGSSPSQSSMVARTQTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVVLETLAG
QRAVKTHGARTKYLKDLVEEEAEEAGVALRSTQSTLQAGLAADAWAAPIAMQIYKKHLDP
RPGPCPPELGLGLGQLACCCLHRRAKRRPPMTQVYERLEKLQAVVAGVPGHLEAASCIPP
SPQENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALR
SWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPPQI
IINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS
Human IRAK1 nucleotide sequence
atggccgggg ggccgggccc gggggagccc gcagcccccg gcgcccagca
cttcttgtac gaggtgccgc cctgggtcat gtgccgcttc tacaaagtga
tggacgccct ggagcccgcc gactggtgcc agttcgccgc cctgatcgtg
cgcgaccaga ccgagctgcg gctgtgcgag cgctccgggc agcgcacggc
cagcgtcctg tggccctgga tcaaccgcaa cgcccgtgtg gccgacctcg
tgcacatcct cacgcacctg cagctgctcc gtgcgcggga catcatcaca
gcctggcacc ctcccgcccc gcttccgtcc ccaggcacca ctgccccgag
gcccagcagc atccctgcac ccgccgaggc cgaggcctgg agcccccgga
agttgccatc ctcagcctcc accttcctct ccccagcttt tccaggctcc
cagacccatt cagggcctga gctcggcctg gttccaagcc ctgcttccct
gtggcctcca ccgccatctc cagccccttc ttctaccaag ccaggcccag
agagctcagt gtccctcctg cagggagccc gcccctctcc gttttgctgg
cccctctgtg agatttcccg gggcacccac aacttctcgg aggagctcaa
gatcggggag ggtggctttg ggtgcgtgta ccgggcggtg atgaggaaca
cggtgtatgc tgtgaagagg ctgaaggaga acgctgacct ggagtggact
gcagtgaagc agagcttcct gaccgaggtg gagcagctgt ccaggtttcg
tcacccaaac attgtggact ttgctggcta ctgtgctcag aacggcttct
actgcctggt gtacggcttc ctgcccaacg gctccctgga ggaccgtctc
cactgccaga cccaggcctg cccacctctc tcctggcctc agcgactgga
catccttctg ggtacagccc gggcaattca gtttctacat caggacagcc
ccagcctcat ccatggagac atcaagagtt ccaacgtcct tctggatgag
aggctgacac ccaagctggg agactttggc ctggcccggt tcagccgctt
tgccgggtcc agccccagcc agagcagcat ggtggcccgg acacagacag
tgcggggcac cctggcctac ctgcccgagg agtacatcaa gacgggaagg
ctggctgtgg acacggacac cttcagcttt ggggtggtag tgctagagac
cttggctggt cagagggctg tgaagacgca cggtgccagg accaagtatc
tgaaagacct ggtggaagag gaggctgagg aggctggagt ggctttgaga
agcacccaga gcacactgca agcaggtctg gctgcagatg cctgggctgc
tcccatcgcc atgcagatct acaagaagca cctggacccc aggcccgggc
cctgcccacc tgagctgggc ctgggcctgg gccagctggc ctgctgctgc
ctgcaccgcc gggccaaaag gaggcctcct atgacccagg tgtacgagag
gctagagaag ctgcaggcag tggtggcggg ggtgcccggg catttggagg
ccgccagctg catcccccct tccccgcagg agaactccta cgtgtccagc
actggcagag cccacagtgg ggctgctcca tggcagcccc tggcagcgcc
atcaggagcc agtgcccagg cagcagagca gctgcagaga ggccccaacc
agcccgtgga gagtgacgag agcctaggcg gcctctctgc tgccctgcgc
tcctggcact tgactccaag ctgccctctg gacccagcac ccctcaggga
ggccggctgt cctcaggggg acacggcagg agaatcgagc tgggggagtg
gcccaggatc ccggcccaca gccgtggaag gactggccct tggcagctct
gcatcatcgt cgtcagagcc accgcagatt atcatcaacc ctgcccgaca
gaagatggtc cagaagctgg ccctgtacga ggatggggcc ctggacagcc
tgcagctgct gtcgtccagc tccctcccag gcttgggcct ggaacaggac
aggcaggggc ccgaagaaag tgatgaattt cagagc
About Xpress Clones
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
