Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY
RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC
VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL
AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV
REIRQHKLRK LNPPDESGPG CMSCKCVLS
ATGACGGAAT ATAAGCTGGT GGTGGTGGGC GCCGGCGGTG TGGGCAAGAG
TGCGCTGACC ATCCAGCTGA TCCAGAACCA TTTTGTGGAC GAATACGACC
CCACTATAGA GGATTCCTAC CGGAAGCAGG TGGTCATTGA TGGGGAGACG
TGCCTGTTGG ACATCCTGGA TACCGCCGGC CAGGAGGAGT ACAGCGCCAT
GCGGGACCAG TACATGCGCA CCGGGGAGGG CTTCCTGTGT GTGTTTGCCA
TCAACAACAC CAAGTCTTTT GAGGACATCC ACCAGTACAG GGAGCAGATC
AAACGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGCTGG TGGGGAACAA
GTGTGACCTG GCTGCACGCA CTGTGGAATC TCGGCAGGCT CAGGACCTCG
CCCGAAGCTA CGGCATCCCC TACATCGAGA CCTCGGCCAA GACCCGGCAG
GGAGTGGAGG ATGCCTTCTA CACGTTGGTG CGTGAGATCC GGCAGCACAA
GCTGCGGAAG CTGAACCCTC CTGATGAGAG TGGCCCCGGC TGCATGAGCT
GCAAGTGTGT GCTCTCCTGA
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
