Human c-MYC Xpress Clone
The MYC protooncogene encodes a multifunctional nuclear phosphoprotein of 65 kDa that can bind DNA and activate or repress transcription (Pesson & leder, 1984). Via this mechanism, MYC regulates expression of numerous target genes that control key cellular functions, including cell growth and cell cycle progression. MYC also has a critical role in DNA replication. Deregulated MYC expression resulting from various types of genetic alterations leads to constitutive MYC activity in a variety of cancers and promotes oncogenesis Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.
OCT3/4, SOX2, c-Myc and KLF4 were initially used to generate induced pluripotent stem cells (iPSC) from mouse adult cells (Takahashi & Yamanaka, 2007). Human somatic cells were later induced with the same set of genes (Park et al., 2008), and with a different set of genes consisting OCT3/4, SOX2, NANOG and LIN28 by Yu et al (2007).
Gene Name: Homo sapiens v-myc myelocytomatosis viral oncogene homolog (avian) (MYC); AKA: bHLHe39; c-Myc; MRTL.
Includes and available separately
| BXS1001-oct: | pMEV2HA-OCT3/4 | 20 ug |
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| BXS1003-sox: | pMEV2HA-SOX2 | 20 ug |
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| BXS1005-nan: | pMEV2HA-NANOG | 20 ug |
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| BXS1007-lin: | pMEV2HA-LIN28 | 20 ug |
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| BXS1009-klf: | pMEV2HA-KLF4 | 20 ug |
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| BXS1011-cmyc: | pMEV2HA-cMYC | 20 ug |
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Custom size is also available upon request.
Specifications
| Materials Provided: | 20 ug transfection ready plasmids in 40ul TE buffer. |
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| Antibiotic Selections: | Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells |
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| Cloning vector: | pMEV-2HA |
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| 5' cloning site: | Bam HI |
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| 3' cloning site: | Xba I |
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| GenBank#: | NM_002467 |
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| Mutations: | None |
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Maintenance of plasmid clones
Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. If received in lyophilized form, the plasmid can be reconstituted in buffer of choice, preferably in 40ul of TE. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
Human cMYC protein sequence
MDFFRVVENQ QPPATMPLNV SFTNRNYDLD YDSVQPYFYC
DEEENFYQQQ QQSELQPPAP SEDIWKKFEL LPTPPLSPSR
RSGLCSPSYV AVTPFSLRGD NDGGGGSFST ADQLEMVTEL
LGGDMVNQSF ICDPDDETFI KNIIIQDCMW SGFSAAAKLV
SEKLASYQAA RKDSGSPNPA RGHSVCSTSS LYLQDLSAAA
SECIDPSVVF PYPLNDSSSP KSCASQDSSA FSPSSDSLLS
STESSPQGSP EPLVLHEETP PTTSSDSEEE QEDEEEIDVV
SVEKRQAPGK RSESGSPSAG GHSKPPHSPL VLKRCHVSTH
QHNYAAPPST RKDYPAAKRV KLDSVRVLRQ ISNNRKCTSP
RSSDTEENVK RRTHNVLERQ RRNELKRSFF ALRDQIPELE
NNEKAPKVVI LKKATAYILS VQAEEQKLIS EEDLLRKRRE
QLKHKLEQLR NSCA
Human cMYC nucleotide sequence
atggattttt ttcgggtagt ggaaaaccag cagcctcccg cgacgatgcc
cctcaacgtt agcttcacca acaggaacta tgacctcgac tacgactcgg
tgcagccgta tttctactgc gacgaggagg agaacttcta ccagcagcag
cagcagagcg agctgcagcc cccggcgccc agcgaggata tctggaagaa
attcgagctg ctgcccaccc cgcccctgtc ccctagccgc cgctccgggc
tctgctcgcc ctcctacgtt gcggtcacac ccttctccct tcggggagac
aacgacggcg gtggcgggag cttctccacg gccgaccagc tggagatggt
gaccgagctg ctgggaggag acatggtgaa ccagagtttc atctgcgacc
cggacgacga gaccttcatc aaaaacatca tcatccagga ctgtatgtgg
agcggcttct cggccgccgc caagctcgtc tcagagaagc tggcctccta
ccaggctgcg cgcaaagaca gcggcagccc gaaccccgcc cgcggccaca
gcgtctgctc cacctccagc ttgtacctgc aggatctgag cgccgccgcc
tcagagtgca tcgacccctc ggtggtcttc ccctaccctc tcaacgacag
cagctcgccc aagtcctgcg cctcgcaaga ctccagcgcc ttctctccgt
cctcggattc tctgctctcc tcgacggagt cctccccgca gggcagcccc
gagcccctgg tgctccatga ggagacaccg cccaccacca gcagcgactc
tgaggaggaa caagaagatg aggaagaaat cgatgttgtt tctgtggaaa
agaggcaggc tcctggcaaa aggtcagagt ctggatcacc ttctgctgga
ggccacagca aacctcctca cagcccactg gtcctcaaga ggtgccacgt
ctccacacat cagcacaact acgcagcgcc tccctccact cggaaggact
atcctgctgc caagagggtc aagttggaca gtgtcagagt cctgagacag
atcagcaaca accgaaaatg caccagcccc aggtcctcgg acaccgagga
gaatgtcaag aggcgaacac acaacgtctt ggagcgccag aggaggaacg
agctaaaacg gagctttttt gccctgcgtg accagatccc ggagttggaa
aacaatgaaa aggcccccaa ggtagttatc cttaaaaaag ccacagcata
catcctgtcc gtccaagcag aggagcaaaa gctcatttct gaagaggact
tgttgcggaa acgacgagaa cagttgaaac acaaacttga acagctacgg
aactcttgtg cgtaa
Select references
- Persson, H. Leder, P.: Science 225: 718-721, 1984.
- Takahashi K. & Yamanaka S. 2006. Cell, 126(4): 663-76
- Takahashi K. et al., 2007, Cell, 131: 861-72.
- Yu J, et al., 2007, Science, 318: 1917-20
- Park IH, et al., 2008. Nature, 451: 141-6
About Xpress Clones
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
