$678.00Catalog Number: BXP1060-edSize: 20 ug

pMEV2HA-BRAF-ED

Human BRAF Xpress Clones

Genes of the RAF family encode three cytoplasmic serine/threonine protein kinases that are regulated by RAS and mediate cellular responses to growth signals from the cell surface to the nuclear. Human BRAF was first cloned from a testis cDNA library. Mutations of BRAF gene have been found in 66% of melanomas and many other types of cancers. All mutations reside in the kinase domain, with a single substitution, V599E accounting for 80%. K482 is a highly conserved active-site residue. BRAF, and all other kinases, mutated at this position are inactive as a protein kinase. T598 and S601 reside within kinase subdomains VII and VIII and are activating phosphorylation sites. Replacement of these two sites by acidic residues (BRAF-ED) renders BRAF constitutively active.

Gene Name: Homo sapiens v-raf sarcoma viral oncogene homolog B1.

Includes and available separately

BXP1060-4:20 ug each
BXP1060-wt:20 ug
BXP1060-km:20 ug
BXP1060-ed:20 ug
BXP1060-ve:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Xba I
3' cloning site:Sal I
GenBank#:NM_004333
Mutations:K482M; T598E, S601D; V599E

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human BRAF protein sequence

Human BRAF nucleotide sequence

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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