Human AKT1 Xpress Clones
AKT1 and the related AKT2 are serine-threonine protein kinases activated by many growth factors including PDGF, EGF, GFG, IGF-1 and insulin, as well as protein phosphatase inhibitors. This activation is rapid and specific and is mediated by phosphatidylinositol 3-kinase. The activation of AKT1 has also been shown to be essential in the suppression of apoptosis by many of the survival growth factors.
Activation of AKT1 requires the phosphorylation on both Thr-308 and Ser-473. AKT1 can not be activated if either T308 or S473 was changed to an alanine residue. If both T308 and S473 are changed to an aspartic acid residue, however, the kinase become constitutively active. That is, it can activate downstream signaling pathways without any upstream activators. K179 is an essential residue for catalysis and, hence the mutant K179A no longer has protein kinase activity. K179A, T308A/S473A and the mutant with all three mutations have been shown to inhibit the signaling pathways when transfected into mammalian cells that have endogenous AKT1 proteins (i.e. dominant negative effects). These mutants can also been used to identify upstream activators, or to study the phosphorylation patterns of AKT1 in vivo and in vitro.
Includes and available separately
| BXA1010-5: | 20 ug each |
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| BXA1010-wt: | 20 ug |
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| BXA1010-ka: | 20 ug |
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| BXA1010-dd: | 20 ug |
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| BXA1010-aa: | 20 ug |
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| BXA1010-k3a: | 20 ug |
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Custom size is also available upon request.
Specifications
| Materials Provided: | 20 ug transfection ready plasmids in 40ul TE buffer. |
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| Antibiotic Selections: | Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells |
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| Cloning vector: | pMEV-2HA |
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| 5' cloning site: | Bam HI |
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| 3' cloning site: | Kpn I |
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| GenBank#: | NM_005163 |
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| Mutations: | KA: K179A; DD: T308D, S473D; AA: T308A, S473A; KAAA: K179A, T308A, S473A |
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Maintenance of plasmid clones
Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).
Human AKT1 protein sequence
Human AKT1 nucleotide sequence
About Xpress Clones
Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.
