$234.00Catalog Number: BXP1000-1Size: 20 ug

pMEV Mammalian Expression Vector

pMEV Mammalian Expression Vector

-Compact mammalian expression vector
-5' leader optimized under CMV promoter for hight level constitutive protein expression.
-Kanamycin selection marker for bacterial and G418 slection in mammalian cells.
-Ultra pure plastmid, ready for transfection. Custom sizes available.
-No expression tags

 

   
   
   

Custom size is also available upon request.

Specifications

Materials Provided: 20 ug transfection ready plasmids in 40ul TE buffer
Antibiotic Selections: Kan/Neo (bps 2228-3022) for kanamycin selectin in bacteria and G418 selection in mammalian cells
CMV promoter: 1-750 bps
2xHA tag: N/A
Multiple Cloning Site: 835-886
SV40 PolyA signal: 915-1137 bps
f1 origin: 1613-1625 bps
ColE1 origin: 3400-4232 bps
Neo/Kan gene: 2150-2944 bps
T3 RNA polymerase Promotor: 886-907 bps
f1 origin: 1613-1625 bps
   

Maintenance of pMEV vectors

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Circular Map:

 

Map and MCS

 

Multiple Cloning Site:

See Circular Map Section Above 

 

Quantity

$234.00

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