Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8^oC if will be used shortly, or -20 ^oC for long term storage. If received in lyophilized form, the plasmid can be reconstituted in buffer of choice, preferably in 40ul of TE. It is recommended that you propagate the vectors in E. coli strains like DH5α, XL1-Blue, BL21, TG1 or JM109.

• Pick up a single colony and grow up in 10ml of LB plus 50-100 ug/ml ampicillin in a 37^oC shaker for overnight
• Inoculate 1L of LB+50 ug/ml of ampicillin in a 2L flask with the 10ml of overnight culture and grow in the 37^oC shaking incubator
• When the OD600 of the culture reaches 0.6, add 0.2 ml of IPTG stock solution (1.0 M) into the flask and continue shaking at 37^oC for 4 hr
• Harvest the cells by centrifugation at 5000 rpm for 5 min
• The cell pellet can be used immediately for purification of GST-cJun protein. Or it can be frozen at -80^oC for later use