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Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)

Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP509668ESQYeast1mgQuote
EP509668ESQE. coli1mgQuote
BP509668ESQBaculovirus200ugQuote
MP509668ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DF65
Gene NamearoA; Locus:PC1_1733
Protein Name3-phosphoshikimate 1-carboxyvinyltransferase
Region Expressed1-429
Expression Tag6xHis
Purity>90%
AA SequenceMQESLTLQPIKLINGTLNLPGSKSVSNRALLLAALSEGKTRLTNLLDSDDVRHMLTALAA LGVEYHLSSDRTVCEITGLGGAFTASQPLELFLGNAGTAMRPLAAALCLTDGDIVLTGEP RMKERPIGHLVDALRQGGAKIDYLEQENYPPLRLHGGFQGGEISVDGSVSSQFLTALLMT APLAAQDTQISIQGDLVSKPYIDITLHMMKAFGIEVRNENYQRFFVAGRQQYRSPGDYLV EGDASSASYFLAAAAIKGGVVRVTGVGRNSVQGDIRFADVLERMGAIVRWGEDYIECERG ELHAIDMDMNHIPDAAMTIATAALFAQGGTTTLRNIYNWRVKETDRLAAMAIELRKVGAE VEEGNDYIRITPPAKLKAAEIGTYNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFE QLARLSELA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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