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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP386983PZEYeast1mgQuote
EP386983PZEE. coli1mgQuote
BP386983PZEBaculovirus200ugQuote
MP386983PZEMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9301)
UniProt IDA3PCK5
Gene NamehisA; Locus:P9301_08571
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-255
Expression Tag6xHis
Purity>90%
AA SequenceMNLIPAIDLMNGKCVRLFKGDFNKRKDFTKEPHEQAKFWESEGAKCIHIVDLDAAKTGSP TNDKSIKKIAKTVNIPIQIGGGIRSQERIEQLFSYGIEKVIMGTSAIENKELVKDLSNKY PGRIIVGIDAKNGKVSTRGWLEQSNIFATDLVKEFSSFKIASFIVTDINTDGTLEGTNEE FIKSILEITDIPVIASGGVGSISDLLSLVKFENSGLFGVIVGKALYENKFTIKEANNVLS SERLNDFDLNRNYYA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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