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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP383071PZGYeast1mgQuote
EP383071PZGE. coli1mgQuote
BP383071PZGBaculovirus200ugQuote
MP383071PZGMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9515)
UniProt IDA2BW33
Gene NamehisA; Locus:P9515_07851
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-255
Expression Tag6xHis
Purity>90%
AA SequenceMKLIPAIDLMNGKCVRLFKGDFNKRKDFSRKPYEQAKYWEEQGAKCIHIVDLDAAKSGYP SNDQSIKKIAKEVNIPIQIGGGIRSLERIEQLFSYGVDKVIMGTSAIENKELVKNLSTKF PRRIIIGIDAKDGKVSTRGWIEQSDVLATDLVKEFSKFEIASFIVTDINTDGTLEGTNEV FIKKILEITDIPVIASGGVGAISDLLSLTKFEHLGLCGVIVGKALYENKFKISEANNILS PERLQDIPINKDYFA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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