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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP374539NEXYeast1mgQuote
EP374539NEXE. coli1mgQuote
BP374539NEXBaculovirus200ugQuote
MP374539NEXMammalian Cell200ugQuote

Protein Information

SpeciesNeisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18)
UniProt IDA1KSN7
Gene NamehisA; Locus:NMC0573
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFEQGARRLHLVDLNGAFAGVP QNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDVIIGTAAVKNPDFVREACK AFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDDGVNSIIYTDIGRDGMMSGVN IDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGVAGAITGRAIYEGSIDFAQAQQL ADSLD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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