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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP397851BPFYeast1mgQuote
EP397851BPFE. coli1mgQuote
BP397851BPFBaculovirus200ugQuote
MP397851BPFMammalian Cell200ugQuote

Protein Information

SpeciesBradyrhizobium sp. (strain ORS278)
UniProt IDA4YJV4
Gene NamehisA; Locus:BRADO0215
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-244
Expression Tag6xHis
Purity>90%
AA SequenceMILFPAIDLKNGQCVRLEQGDMARATVFNLDPAAQAKSFADQGFEYLHVVDLDGAFAGKP MNAQAVEAMLKIVKMPVQLGGGIRDLKTVEAWLGKGITRVIIGTAAVRDPELVKMAAKAF PGRVAVGLDARDGKVAVEGWAETSEVTALDIAKRFEDAGVAAIIFTDIARDGLLKGLNLD ATIALGDAISIPVIASGGLASIEDVKAMLTPRARKLEGAIAGRALYDGRLDPAEALALIR AISG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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