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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP383200PZFYeast1mgQuote
EP383200PZFE. coli1mgQuote
BP383200PZFBaculovirus200ugQuote
MP383200PZFMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9303)
UniProt IDA2CAH8
Gene NamehisA; Locus:P9303_17461
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-255
Expression Tag6xHis
Purity>90%
AA SequenceMEIIPAIDLLDSACVRLHQGDYAKVTRFSEDPVAQALSWQKQGATRLHLVDLDGAKSGEP VNDSCVRAITSALNIPVQLGGGVRTLERAEELLAYGLEQVILGTVAIEQPQLVKQLAQRN PGRIIVGIDAKNGKVATRGWISQSEVNATDLAADFNAAGIAAIISTDIATDGTLEGPNLE SLRAMANASSVPLIASGGVGCMADLLSLLALEPYGVSGVIVGRALYDGKVDLKEAIRAIG DGRLQDPPSSKPLMA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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