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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP400356CWXYeast1mgQuote
EP400356CWXE. coli1mgQuote
BP400356CWXBaculovirus200ugQuote
MP400356CWXMammalian Cell200ugQuote

Protein Information

SpeciesClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
UniProt IDA5N7R0
Gene NamehisA; Locus:CKL_1299
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-238
Expression Tag6xHis
Purity>90%
AA SequenceMIILPAIDLKGGKCVRLYKGDMNSQEVVARDPFETALKFKSEGAQYLHMVDLDGALKGSG ENLDIISKIVKNVDVPIEVGGGIRNIETIDKFIKLGVSRVILGTAALKNKELVIKAVENY DEKIAVGIDAKDGVPAADGWTTLSKTNYIDFGKEMEKIGVQTLIFTDISKDGTLEGTNLE QLLKLKNSVKCNVIASGGIKDIEDIKSLKKENVYGAIVGKAIYAKTLSLKKAIEIGGN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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