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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP881105HTMYeast1mgQuote
EP881105HTME. coli1mgQuote
BP881105HTMBaculovirus200ugQuote
MP881105HTMMammalian Cell200ugQuote

Protein Information

SpeciesHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
UniProt IDQ9HN14
Gene NamehisA; Locus:VNG_2294G
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-242
Expression Tag6xHis
Purity>90%
AA SequenceMTFESFTVLPAVDMQDGQVVQLVQGERGTERTYGDPVDAATDWVAAGAEALHLVDLDGAF EGARANATAVEDILDATDVSVQVGGGIRSAEDATALLDRGVDRVILGTAAIETPDIVGEI AAAYSDGVLVSLDAKDGEVVVEGWTEGTGMDPVAAAQRYADLGAAGILFTDVDVEGKQEG VRTDPVRDLVDSVDIPVIASGGVATVDDVRALEAAGAAGAVVGTALYEGNFTLADAQAAV ED
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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