1- (5-phosphoribosyl) -->

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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog Number	Expression Host	Amount	In Stock
YP864417NGF	Yeast	1mg	Quote
EP864417NGF	E. coli	1mg	Quote
BP864417NGF	Baculovirus	200ug	Quote
MP864417NGF	Mammalian Cell	200ug	Quote

Protein Information

Species	Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)
UniProt ID	Q9JVH4
Gene Name	hisA; Locus:NMA0839
Protein Name	1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed	1-245
Expression Tag	6xHis
Purity	>90%
AA Sequence	MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFEQGARRLHLVDLNGAFAGVP QNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDVIIGTAAVKNPDFVREACK AFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDDGVNSIIYTDIGRDGMMSGVN IDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKRGVAGAITGRAIYEGSIDFAQAQQL ADSLD
Storage Buffer	PBS pH 7.4, 50% glycerol
Storage	Store working aliquots at 4^oC for upto a week. Freeze at -20 to -80^oC for long term storage. Avoid repeated freeze/thaw.

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