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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP886507TMIYeast1mgQuote
EP886507TMIE. coli1mgQuote
BP886507TMIBaculovirus200ugQuote
MP886507TMIMammalian Cell200ugQuote

Protein Information

SpeciesThermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
UniProt IDQ9RPQ5
Gene NamehisA
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-237
Expression Tag6xHis
Purity>90%
AA SequenceMIIYPAVDIKDGRCVRLVQGEFDKVTVYSDNPVEMGLKWERMGAQYLHVVDLDGARTGQI QNTPIISEMAVKLGIPVQLGGGIRTVETIETLLCKGIHRVILGTSAVKNPELVKQALKTF EDSVVIGIDAKDGMVAIEGWAKTSEFTAIRFAKKMEELGAKTIIYTDISRDGMLAGPNLK AMEEMVKAVNIEVIASGGVRNIDDIRNLKNVGVSGVIVGKALYTGDLDLKEAIEVAK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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