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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP862675VEXYeast1mgQuote
EP862675VEXE. coli1mgQuote
BP862675VEXBaculovirus200ugQuote
MP862675VEXMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
UniProt IDQ9KSW9
Gene NamehisA; Locus:VC_1137
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMIIPALDLIEGQVVRLYQGDYGQVTEYKVDPAEQFNLYHQAGAGWLHLVDLTGAKNTAAR QLNLIARLLASTPANIQIGGGVRSEQDVVDLLEAGAQRVVVGSTAVKQPQLVKSWIEKYG AEKIVLALDINIDEQGTRKVAISGWQEDSGVTIEALIDDYLTVGLQHVLCTDISRDGTLA GSNVELYRDLCRQYPQVQFQSSGGIGSLADIEALKGTGVAGVIVGRALLDGKFTAQEAFA CWQNG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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