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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP742543EYOYeast1mgQuote
EP742543EYOE. coli1mgQuote
BP742543EYOBaculovirus200ugQuote
MP742543EYOMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9313)
UniProt IDQ7TV18
Gene NamehisA; Locus:PMT_0520
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-255
Expression Tag6xHis
Purity>90%
AA SequenceMEIIPAIDLLDSVCVRLHQGDYEKVTRFSEDPVDQALSWQKQGATRLHLVDLDGAKSGEP VNDSCVRAITSALNIPVQLGGGVRTLERAEELLAYGLEQVILGTVAIEQPQLVKQLAQRN PGRIIVGIDAKNGKVATRGWISQSEVNATDLASDFNAAGIAAIISTDIATDGTLEGPNLE SLRAMANASSVPVIASGGVGCMADLLSLLALEPYGVSGVIVGRALYDGKVDLKEAIRAIG DGRLQDPPTSKPLMA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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