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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP815328BMQYeast1mgQuote
EP815328BMQE. coli1mgQuote
BP815328BMQBaculovirus200ugQuote
MP815328BMQMammalian Cell200ugQuote

Protein Information

SpeciesBrucella suis biovar 1 (strain 1330)
UniProt IDQ8FY08
Gene NamehisA; Locus:BR2084, BS1330_I2078
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-243
Expression Tag6xHis
Purity>90%
AA SequenceMILFPAIDLKDGQCVRLKLGDMDQAIIYNEDPAAQAKAFEDQGFEWLHVVDLNGAFAGES VNGTAVEAILKATKNPVQLGGGIRTLAHIENWLSRGLRRVILGTVAVRDPALVMEACKAF PGQVAVGIDAKGGKVAVEGWAEASRLGVIELAKKFEGAGVAAIIYTDIDRDGVLAGINWD STLALAEAVSIPVIASGGLASMEDIRRLATPEMRKLEGAISGRALYDGRIDPAEALSVLR AAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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