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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP763486HCOYeast1mgQuote
EP763486HCOE. coli1mgQuote
BP763486HCOBaculovirus200ugQuote
MP763486HCOMammalian Cell200ugQuote

Protein Information

SpeciesHelicobacter hepaticus (strain ATCC 51449 / 3B1)
UniProt IDQ7VHY5
Gene NamehisA; Locus:HH_0826
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-243
Expression Tag6xHis
Purity>90%
AA SequenceMALDIYPAIDLKEGKAVRLFKGDMQSATIYGEALEFAKMFEDMGAKWLHIVDLDGAFAGI PQNLKYIEEILRTTHLQIQIGGGIRNEERIRLYMDLGVSRVILGSIAIKNWEFVKTMAHS YPIAVGIDAREGKVAVQGWAKESDICSSVLAEKFAGSEVQAIICTDINRDGALSGINVSF TQDIACRSGIYTIASGGFASSNELEILDENPYISGVIIGKAFYEGKINLKEALALFENKK VKI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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