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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (his-6)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (his-6) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP742408NHAYeast1mgQuote
EP742408NHAE. coli1mgQuote
BP742408NHABaculovirus200ugQuote
MP742408NHAMammalian Cell200ugQuote

Protein Information

SpeciesNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
UniProt IDQ7RXQ8
Gene Namehis-6; ORFs:NCU00150
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-257
Expression Tag6xHis
Purity>90%
AA SequenceMTRFRPCIDLHAGQVKQIVGGTLDSATSELRTNFVSPHPPAYFAKLYRDNDLSGAHVIML GPGNKEAALESLKAWPGGLQVGGGITDANAREWVEAGAEKVIITSYLFPNGKFSQSHLDA VLAALDGDKSKLVIDLSCRRQGDDRWFVAMNKWQTITDMEVSEESIKALEPYCSEFLIHA ADNEGLQKGIDEKLVQRLSEWCSIPVTYAGGGRNLEDLETVKRLSGGKVDLTIGSALDCF GGKGVTLQECVEWNRRQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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