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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase 2 (hisA2)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase 2 (hisA2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP766233PIJYeast1mgQuote
EP766233PIJE. coli1mgQuote
BP766233PIJBaculovirus200ugQuote
MP766233PIJMammalian Cell200ugQuote

Protein Information

SpeciesPhotorhabdus luminescens subsp. laumondii (strain TT01)
UniProt IDQ7N8D1
Gene NamehisA2; Locus:plu0797
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase 2
Region Expressed1-258
Expression Tag6xHis
Purity>90%
AA SequenceMISKETFEIFPSVDIVDGIAMTSNSAIRLTRGESSTLRRPLGTPAEVALAWQNAGAKWIH VVDLDAASGRGSNSHLIADVLAAVDINVQVCGGIRDETILRKVLATGCQRVNLGTAALEN PDWCAQAIAEYGDKIAIALDVKSTSEGYQLATHGWNVSKGNLWDLLERLDKQGCQRYVVT DVERGGMMSSPNFTLLQEVCAKTSSPVIAGGGVSSLDDLRALADLSTIGVEGAILGQALH IGKVPLEDALAATQSPRR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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