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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP722754HTJYeast1mgQuote
EP722754HTJE. coli1mgQuote
BP722754HTJBaculovirus200ugQuote
MP722754HTJMammalian Cell200ugQuote

Protein Information

SpeciesHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
UniProt IDQ5UZH1
Gene NamehisA; Locus:rrnAC2529
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-246
Expression Tag6xHis
Purity>90%
AA SequenceMYPEFEVVPAVDMQDGQVVQLVGGERGTEKTYGDPVEAAQRWVDAGARTLHLVDLDGAFE GERQNAAAIDAVLDAVGADVDVQLGGGIRTAEDAVSLLDRGLDRVILGTAAVETPEIVGE ISDEHPGSVLVSLDAKDGEVVVSGWTEGTGLDPADAAGRYADLGAGGILFTDVDVEGQLD GVRTDPVRRLVDSVDIPVIASGGVATINDVLALRSAGAAAVVVGSALYEGQFTLDAAIDA LGESSE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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