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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP713373Yeast1mgQuote
EP713373E. coli1mgQuote
BP713373Baculovirus200ugQuote
MP713373Mammalian Cell200ugQuote

Protein Information

SpeciesBacillus clausii (strain KSM-K16)
UniProt IDQ5WDI1
Gene NamehisA; Locus:ABC3045
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-240
Expression Tag6xHis
Purity>90%
AA SequenceMSYTIYPAIDIRAGKCVRLEQGDYNKETIYGESPYKMAKSFQDAGAKWVHVVDLDGAKAK RPVNDEAILTIAQKLDVPVQVGGGIRTEQDIDRYINGGVARVVLGSVAVYQPDFTKQMLA KYGEQMAIGIDARDGYVATEGWLETSHVKAEDLAAEMARFGAKTFIFTDIARDGMLTGPN IEACVALSKAANANVIASGGVSSIADLAQLRAASLAGAIVGKALYTKRFTLEEALAEGEL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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