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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP739369AWWYeast1mgQuote
EP739369AWWE. coli1mgQuote
BP739369AWWBaculovirus200ugQuote
MP739369AWWMammalian Cell200ugQuote

Protein Information

SpeciesAcinetobacter sp. (strain ADP1)
UniProt IDQ6F7A5
Gene NamehisA; Locus:ACIAD3398
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-243
Expression Tag6xHis
Purity>90%
AA SequenceMLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVATAQHWVNEGARRLHLVDLNGAFAGTP IHKPVVEAIAKAQPELPIQIGGGIRSLETIEHYLDAGVSFVIIGTKAVQDPEFVEQACKQ FAGHIIVGIDAKDGMVATDGWANVTDVKATDLAKRFADAGVSSIVYTDIARDGMMQGVNV EQTVNLAQYSGLPVIASGGVTNLDDVRLLKGKPGILGAITGRAIYEGTLSLREAQLLLDQ NTL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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